Low transcriptional activity and poor metabolic control limit the production of target compounds in microbial hosts. Increasing expression efficiency overcomes bottlenecks in industrial fermentation.

Firmicutes and related industrial strains often exhibit poor DNA uptake and conjugation competence. Overcoming this barrier allows for more efficient genetic engineering and metabolic pathway optimization.

Inefficient separation of proteins from complex fermentation mixtures leads to significant product loss. Improving recovery efficiency increases the economic viability of large-scale microbial manufacturing.