The keywords focus on compositions and methods for producing tagatose, indicating that current natural extraction or chemical synthesis is inefficient. Improving the biocatalytic conversion efficiency addresses the bottleneck of high production costs and low output volume.

The keywords focus on the production of specific steviol glycosides like Rebaudioside D and M, which are naturally scarce. The primary technical bottleneck is the inefficient enzymatic conversion and low throughput of traditional glycosylation processes.

The keywords highlight the need for motifs with low side reactivity and specific phosphatases to prevent unwanted byproducts. Improving specificity ensures higher yields of the target rare sugar without contamination from competing metabolic pathways.