Bacillus strains often fail to colonize the gut effectively enough to suppress E. coli infections and maintain growth performance.

Standard amylase enzymes denature at low gastric pH, preventing effective glucose yield and starch utilization in the small intestine.

Non-invasive fecal sampling often fails to accurately quantify internal microbial populations and metabolic health indicators in poultry.

Combining direct-fed microbials with exogenous proteases or phytases can lead to antagonistic interactions that reduce nutrient digestibility.