High temperatures during amplification cycles cause structural instability and loss of catalytic activity in standard enzymes. Preventing this degradation ensures consistent molecular detection and pathogen control.

Agricultural yields are limited by uncontrolled canker disease and microbial inactivity in extreme saline-alkali or low-temperature environments. Overcoming these biological constraints ensures consistent biocontrol and nutrient availability under harsh conditions.

Enzymatic degradation and loss of activity during industrial processing. Enhancing structural integrity prevents premature catalyst inactivation.