Standard surfactant systems exhibit reduced kinetic energy and solubility in cold water, leading to poor grease emulsification. Improving cold cleaning performance addresses the energy-intensive limitation of requiring heated water for effective sanitation.

Standard fermentation processes often result in low titers or undesirable mixtures of mono- and di-rhamnolipids. Increasing specific mono-rhamnolipid output improves surfactant performance consistency and reduces downstream purification costs.