Enzyme inactivation from pH reduction during degradation 55 documents (11%) Enzyme Engineering & Protein Optimization
High polymer crystallinity hindering enzyme accessibility 36 documents (7%) Process Engineering & Environmental Control
Low metabolic efficiency of single enzyme systems 28 documents (5%) Microbial & Metagenomic Discovery
Substrate inhibition from intermediate BHET accumulation 27 documents (5%) Microbial & Metagenomic Discovery
Inefficient breakdown of mixed polyolefin waste streams 22 documents (4%) Microbial & Metagenomic Discovery
Substrate inhibition during high concentration plastic depolymerization 20 documents (4%) Enzyme Engineering & Protein Optimization
Inefficient monomer separation from reaction media 20 documents (4%) Process Engineering & Environmental Control
Crystalline polymer resistance to enzymatic hydrolysis 20 documents (4%) Process Engineering & Environmental Control
Plastic persistence due to lack of embedded catalysts 18 documents (3%) Advanced Delivery & Enzyme Complexes
Low catalytic activity at ambient temperatures 16 documents (3%) Process Engineering & Environmental Control
Low thermal stability of native IsPETase enzymes 15 documents (3%) Enzyme Engineering & Protein Optimization
Contamination from blended textile fiber waste 15 documents (3%) Process Engineering & Environmental Control
Slow degradation rates of synthetic polyester waste 15 documents (3%) Advanced Delivery & Enzyme Complexes
Low PET degradation rates from isolated enzyme activity 15 documents (3%) Advanced Delivery & Enzyme Complexes
Selective polyurethane recovery from multilayer packaging waste 14 documents (3%) Non-Polyester & Complex Polymer Degradation